Journal: Neuron

Article Title: Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors

doi: 10.1016/j.neuron.2019.01.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Authentication of cell lines used We used HEK293T cells (sex typed as female) and H9 ESC (sex typed as female) that were obtained directly from ATCC, and thus they were not further authenticated.

Techniques: Virus, Recombinant, Mutagenesis, SYBR Green Assay, shRNA, Software

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: MV411 cells express C-type lectin-like molecule-1 (CLL1). A) Confocal microscopy of MV411 cells stained for DAPI, CLL1, and actin. Scale bar = 3 μm. B) Histogram showing expression of CLL1 receptor in MV411 cells as detected by flow cytometry. Black = unstained MV411 cells, blue = CLL1-antibody treated MV411 cells.

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Confocal Microscopy, Staining, Expressing, Flow Cytometry

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: Unmodified PSMA-b-PS NP MV411 are cytocompatible and are taken up in a concentration dependent manner. A) Treatment of MV411 cells with untargeted PSMA-b-PS NPs for 2 and 24 h normalized to the no treatment (NT) group. Two-way ANOVA followed by Dunnett’s multiple comparisons was used to compare NT group with each concentration at all-time points (*p < 0.05). B) Percent of NP positive MV411 cells after treating with increasing concentrations of untargeted PSMA-b-PS NPs. Data represents mean ± standard error mean (N = 3, n = 3). Two-way ANOVA followed by Sidak’s multiple comparisons was used to compare 2 and 24 h time points (****p < 0.0001). C,D) Representative histogram of uptake at 2 and 24 h. E) Median fluorescence intensity (MFI) normalized to no treatment. Data represents mean ± standard (n = 9). Two-way ANOVA followed by Sidak’s multiple comparisons was used to compare 2 and 24 h time points (****p < 0.0001).

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Concentration Assay, Fluorescence

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: Multivalent presentation of cCBP on PSMA-b-PS NPs does not increase NP uptake in MV411 cells. A) Schematic showing cCBP incorporation on NPs. B) Functionalization efficiency of cCBP-NPs. C) Representative transmission electron microscopy (TEM) images of untargeted NP, cCBP200-NP, and CBP30000-NPs. Scale bar = 100 nm. D) Percent of cells positive for cCBP-NPs after incubation for 2 and 24 h in 37 °C. E) Median fluorescent intensity (MFI) normalized to untreated cells of cCBP-NPs after incubation for 2 and 24 h in 37°C. Data represents mean ± standard error mean (n = 9). Two-way ANOVA and Dunnett’s multiple comparisons indicate *p < 0.05 versus untargeted NP.

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Transmission Assay, Electron Microscopy, Incubation

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: Percent of NP positive MV411 cells is reduced when monoclonal CLL1 antibody is used to block the receptor. A) Percent of NP positive cells after treatment with CLL1-mAb to block prior to NP incubation. B) Percent of NP positive cells after treatment with free cCBP to block prior to NP incubation. Data represents mean ± standard error mean (n = 6). Two-way ANOVA followed by Sidak’s multiple comparisons was used to compare treatment without blocking to CLL1-mab and cCBP groups, respectively (*p < 0.001, ns = not significant).

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Blocking Assay, Incubation